epsN [2018-09-20 18:42:44]

UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase, required for extracellular polysaccharide synthesis

locus: BSU34230

pI: 5.84

mw: 42.76 kDa

protein length: 388 aa

gene length: 1164 bp

function: biofilm formation

product: UDP-2,6-dideoxy 2-acetamido 4-keto glucose aminotransferase

essential: no

synonyms: yvfE

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.1|Matrix polysaccharide synthesis]

Gene

Coordinates: 3,515,062 → 3,516,228

Phenotypes of a mutant

• smooth colonies on MsGG medium, no [SW|biofilm formation] [Pubmed|22113911]

The protein

Catalyzed reaction/ biological activity

• conversion of UDP-2,6-dideoxy 2-acetamido 4-keto glucose to UDP-2,6-dideoxy 2-acetamido 4-amino glucose [pubmed|29982582]

Protein family

• degT/dnrJ/eryC1 family (according to Swiss-Prot)

[SW|Cofactors]

• pyridoxal phosphate [pubmed|29982582]

Expression and Regulation

Operons

• genes: [gene|F6C4634BF871D9A01E27014FFED7527C54BF560D|epsA]-[gene|9B668909E1380B287ACE58561DCF45FC29184D8B|epsB]-[gene|5DB168A3D087AAEB003D7548A1A4356ABD07F712|epsC]-[gene|223E979276F07D69D0DDAEE906023A8AB4F37F8E|epsD]-[gene|5D717F9F54693FD0AA8BC49E10348637858F88B9|epsE]-[gene|DB47BA2A5E3BED51DD8630E7D321C099D74B46CF|epsF]-[gene|C4D81976AAE888C4FE89A2EE5CF9A763CAD645FF|epsG]-[gene|BD843389F95BFB905071547AB243413A617F12A8|epsH]-[gene|6EBD8F7065B6C1996067F3120DD9CFEA91D3A444|epsI]-[gene|5272E807B6B98541EA155AA9748320E25767F096|epsJ]-[gene|66076952E512D53FC1FC62455A86ACFA21AE120D|epsK]-[gene|D67665412B78B56752B460219445E4BEF52C3A1A|epsL]-[gene|40EA584CF77FFF7D7E4E74B414EC69ADE3A1585B|epsM]-[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]-[gene|52A47B49B1FB955EE7E3C316B57A2B4134F78480|epsO]
  description: [Pubmed|15661000]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15661000], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]: activation, [Pubmed|23646920], in [regulon|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA regulon]
  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]
  • [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: anti-activation, (prevents binding of [protein|6AF1CB8998BE9C71DF4F1201A7F74FF7E75CB799|RemA]) [Pubmed|23646920], in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]
  • [regulon|EAR riboswitch|EAR riboswitch]: processive antitermination, in [regulon|EAR riboswitch|EAR riboswitch]

  regulation

  • repressed by [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] [Pubmed|15661000]

  additional information

  • induction by sequestration of [protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR] by [protein|24D6A7DDCB25B99FBB670E9203F826596916B35C|SinI] or [protein|C17C296F3EB2E106C380E3B5D784F3FA63F4C9B7|SlrA] [PubMed|15661000,19788541]
  • expression is increased in [gene|21D260E900776EAAF4B6000A3365DCA9241EACA6|rsiX] mutants [pubmed|32483306]

Biological materials

Mutant

• MGNA-A064 (yvfE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/64 NBRP B. subtilis, Japan]
• BKE34230 (Δ[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA, downstream forward: _UP4_TTCCATACTGTCGAGGTGAA
• BKK34230 (Δ[gene|0D24AB8D446CFFBC6DEAD3C10D1FA59551545FE8|epsN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TAAGTAGATTTTTTTATGCA, downstream forward: _UP4_TTCCATACTGTCGAGGTGAA

Labs working on this gene/protein

• [SW|Richard Losick], Harvard Univ., Cambridge, USA [http://www.mcb.harvard.edu/Losick/ homepage]

References

Reviews

• 20735481

Research papers

• 29982582

=The EAR [SW|RNA switch]=

• 20374491,20230605